Recommended reaction scheme: T-line: Immobilized streptavidin;
Conjugate Pad: Antibody1-conjugate; Sample Pad: Antibody2-biotin
News & Blogs 2023-10-30
The Potential of the Biotin-Avidin System (BAS) in Immunoassays
What is the Biotin-Avidin—System(BAS)？
The Biotin-Avidin System (BAS) is a widely used signal amplification system in immunoassays. At its core, Avidin, a common glycoprotein, consists of four identical subunits, each equipped with a biotin-binding site, making it exhibit a strong affinity for biotin.
Streptavidin, composed of four identical peptide chains, allows one Streptavidin molecule to bind to four biotin molecules. This binding property leads to an amplification effect, often employed as an intermediary in immunoanalyses.
One of the most prevalent applications involves immobilizing Avidin on the surface of magnetic beads while labeling antibodies with biotin. The high affinity between biotin and Avidin allows antibodies to be indirectly adsorbed onto the solid phase through this interaction.
Streptavidin molecules can also aggregate through non-covalent interactions, forming dimers, tetramers, and large aggregates.
As a result, the Avidin-biotin system can significantly amplify the signal response in immunoassays.
Application Platforms
Poly-SA: high molecular weight is more suitable for larger particle/solidphase.
SA-biotin System Used in Sandwich Immunoassays:
The T-line is immobilized with streptavidin;
Conjugate Pad: Antibody1-conjugate; Sample Pad: Antibody2-biotin
SA-biotin System Applied in Lateral Flow:
Advantages of the Antigen, Antibody and Active protein(Streptavidin) provided in BIOEAST：
1. Tested on the immunoassay platform within the facility, yielding good results.
2. Corresponding conjugation conditions are available, reducing optimization costs for various projects.
3. Enhances signal and sensitivity.
4. Exhibits good stability.
5. Reduces interference from biotin.
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