News & Blogs 2023-11-27
Introduction for Use | BIOEAST Colored Latex Microspheres
Intended use
BIOEAST Colored Latex Microspheres are prepared by staining polystyrene latex microspheres using a unique deep dyeing technique. The colors are vibrant, and the dye is evenly distributed inside the microspheres, with very little adsorption on the surface or shedding into the dispersion. This product series is available in various colors, including red, blue, and black, and can provide a wide range of customized particle size products for use in lateral flow and latex agglutination tests.
Features
Unique deep dyeing technology for rich, vibrant colors
Dye is confined to the interior, the surface clean, no dye particles in the dispersion
Highly uniform particle size, CV ≤ 3%, low batch-to-batch variation
Information
Solid Content: 4%
Dispersion Medium: Purified water with trace amounts of surfactant
Content: 50~200 μmol/g, customizable
Particle Sizes: 200 nm, 300 nm, 400 nm, customizable
Antibody Conjugation Method Example
Recommended Buffer
| Name | Description | Preparation |
| MES | 50 mM MES，pH 6.0 | 1.06 g MES monohydrate (CAS: 145224-94-8) dissolved in 80 mL purified water，adjust to pH 6.0 and adjust to 100 mL. |
| Sulfo-NHS Solution | 10 mg/mL in MES | 100 mg sulfo-NHS (CAS: 145224-94-8) dissolved in 10 mL MES buffer. |
| EDC Solution | 10 mg/mL in MES | 100 mg EDC (CAS: 25952-53-8) dissolved in 10 mL MES buffer. |
| Blocking Solution | 50 mM HEPES，1% BSA，pH 8.0 | 1.19 g HEPES (CAS: 7365-45-9) dissolved in 80 mL purified water，adjust to pH 8.0 and adjust to 100 mL. Dissolve 1.0 g BSA (CAS: 9048-46-8) in the buffer. |
Experimental Steps
Washing: Take 120 μL color latex microspheres (4% solid content), disperse with an ultrasonic cell crusher, centrifuge (centrifugal force > 15,000) for 5 minutes, remove the supernatant, and wash with MES by centrifugation once.
Activation: Add 300 μL MES, cool on crushed ice, add 40 μL sulfo-NHS solution, shake well, then add 20 μL EDC solution, shake well and react at room temperature for 20 minutes. Centrifuge (centrifugal force > 15,000) for 5 minutes, remove the supernatant, and wash with MES by centrifugation once.
Antibody Conjugation: Add 480 μL MES, disperse with ultrasound, add 160 μg antibody, shake well, and react at room temperature for 4 hours. Centrifuge (centrifugal force > 15,000) for 5 minutes, remove the supernatant.
Blocking: Add 480 μL blocking solution, disperse with ultrasound, and react at room temperature for 1 hour. Centrifuge (centrifugal force > 15,000) for 5 minutes, remove the supernatant, and wash with blocking solution by centrifugation once.
Storage: Add 480 μL storage solution (same as the blocking solution, with a small amount of stabilizer), disperse with ultrasound, and store at 2~8 °C.
Product List
| Red Polystyrene | DR0200C | Carboxyl Group(COOH) | 200nm | 4% | LF |
| DR0300C | Carboxyl Group(COOH) | 300nm | 4% | LF | |
| DR0400C | Carboxyl Group(COOH) | 400nm | 4% | LF | |
| DR0200S | Streptavidin(SA) | 200nm | 1% | LF | |
| DR0300S | Streptavidin(SA) | 300nm | 1% | LF | |
| DR0400S | Streptavidin(SA) | 400nm | 1% | LF | |
| Blue Polystyrene | DB0200C | Carboxyl Group(COOH) | 200nm | 4% | LF |
| DB0300C | Carboxyl Group(COOH) | 300nm | 4% | LF | |
| DB0400C | Carboxyl Group(COOH) | 400nm | 4% | LF | |
| DB0200S | Streptavidin(SA) | 200nm | 1% | LF | |
| DB0300S | Streptavidin(SA) | 300nm | 1% | LF | |
| DB0400S | Streptavidin(SA) | 400nm | 1% | LF | |
| Black Polystyrene | DK0200C | Carboxyl Group(COOH) | 200nm | 4% | LF |
| DK0300C | Carboxyl Group(COOH) | 300nm | 4% | LF | |
| DK0400C | Carboxyl Group(COOH) | 400nm | 4% | LF | |
| DK0200S | Streptavidin(SA) | 200nm | 1% | LF | |
| DK0300S | Streptavidin(SA) | 300nm | 1% | LF | |
| DK0400S | Streptavidin(SA) | 400nm | 1% | LF |