News & Blogs 2023-12-13
Introduction for Use | BIOEAST Europium Chelated Polystyrne Microspheres (Pink)
Introductions
Intended use
Bioeast Europium Chelated Polystyrene Microspheres (pink) are polystyrene microspheres infused with europium chelate fluorescent dye. Bioeast's unique dye-loading technology immobilizes the europium chelate molecules within the microspheres, ensuring high fluorescence intensity while maintaining a clean microsphere surface. With a Stokes Shift exceeding 250 nm, Bioeast Europium Chelated Polystyrene Microspheres effectively minimize background interference. These microspheres also feature an appropriate concentration of carboxyl groups on their surface, facilitating easy covalent coupling with antibodies or active substances containing amino groups. This enables the creation of stable microsphere-ligand complexes widely used in fluorescent immunochromatographic reagents.
Features
- Uniform particle size, minimal batch-to-batch variation
- High dye loading, resulting in intense fluorescence
- Single batch capacity exceeding 10L to meet various production needs
- Customized services available
Parameters
- Particle size: 100 nm, 200 nm, 300 nm
- Homogeneity: Coefficient of Variation (CV) ≤3%
- Solid content: 1.0% w/v
- Surface functional group: Carboxyl group (COOH)
- Dispersion system: Purified water, trace surfactant, 0.05% ProClin 300
- Excitation wavelength (λ(Ex)): 365 nm
- Emission wavelength (λ(Em)): 610 nm
Precautions
It is recommended to store this product series between 2 °C and 8 °C, away from light, in an airtight container, and avoid freezing. Before usage, it is advised to disperse the microspheres through ultrasonic or vortex mixing to ensure uniform dispersion.
One-step labelling protocol
Solution Preparation
| NAME | PREPARATION | |
| MES | 50 mM MES，pH 6.0 | |
| Sulfo-NHS solution | 10 mg/mL in MES | |
| EDC Solution | 10 mg/mL in MES | |
| Blocking Solution | 50 mM HEPES，1% BSA，0.1% ProClin 300，pH 8.0 | |
Suggested labelling protocol
Activation:
Dilute 100 μL of Bioeast time-resolved latex microspheres (1% solid content) with 900 μL of MES, disperse using ultrasound. Add 12 μL of Sulfo-NHS solution, shake thoroughly. Then, introduce 10 μL of EDC solution, shake well, and allow it to react at room temperature for 20 minutes. Centrifuge (with a centrifugal force > 15,000) for 5 minutes, discard the supernatant, and conduct one centrifugation wash with MES.
Antibody Conjugation:
Disperse the obtained microspheres in 200 μL of MES using ultrasound. Add 120 μg of the antibody (previously diluted to 300 μL with MES), shake well, and let it react at room temperature for 2 hours.
Blocking:
Incorporate 50 μL of the blocking solution, disperse with ultrasound, and allow it to react at room temperature for at least 1 hour (overnight blocking is possible). Centrifuge (with a centrifugal force > 15,000) for 2 minutes, discard the supernatant, and conduct one wash with the blocking solution through centrifugation.
Storage:
Add 100 μL of the storage solution, disperse with ultrasound, and store at 2 °C to 8 °C.
Product List
| PRODUCT NAME | CAT NO. | SURFACE GROUP | SIZE | CONCENTRATION | APPLICATION PLATFORM |
| Europium Chelated Polystyrne Microspheres (pink) | EU0100C | Carboxyl Group(COOH) | 100nm | 1% | FIA |
| EU0200C | Carboxyl Group(COOH) | 200nm | 1% | FIA | |
| EU0300C | Carboxyl Group(COOH) | 300nm | 1% | FIA | |
| EU0100S | Streptavidin(SA) | 100nm | 1% | FIA | |
| EU0200S | Streptavidin(SA) | 200nm | 1% | FIA | |
| EU0300S | Streptavidin(SA) | 300nm | 1% | FIA |